Fig. 4
From: Utility of the pat gene as a selectable marker gene in production of transgenic Dunaliella salina
Screening and PCR analysis of transformants. a Selection of colonies of individual-transformed cells on a Basta®-containing agar plate. The single colonies of bombarded cells containing the pat plasmid appeared. b Identification of transformed D. salina with PCR. M, DNA marker; B, 1 × TE; lanes 1–6, PCR products from transformed D. salina cells; lane P, PCR product from pat plasmid; lane N, PCR product from wild-type D. salina cells