Antioxidant and angiotensin I-converting enzyme inhibitory activities of northern shrimp (Pandalus borealis) by-products hydrolysate by enzymatic hydrolysis

Table 5 Antioxidant and ACE inhibitory activities for the molecular weight cut-off fractions of the protamex hydrolysate

Sample	ABTS⁺ scavenging activity	SOD-like activity	Reducing power	ACE inhibitory activity
Sample	IC₅₀ (mg/mL)¹	IC₅₀ (mg/mL)	EC₅₀ (mg/mL)²	IC₅₀ (mg/mL)
< 3 K	0.22 ± 0.01^{3, b}	>10	20.14 ± 0.39^a	0.06 ± 0.00^a
3–10 K	0.22 ± 0.00^b	>10	13.84 ± 0.16^b	0.03 ± 0.00^b
> 10 K	0.24 ± 0.01^a	>10	7.04 ± 0.83^c	0.03 ± 0.00^b
l-Ascorbic acid^A	0.005 ± 0.000^c	0.07 ± 0.00^d	0.04 ± 0.00^d
Captopril^B				0.00001 ± 0.0000^c

Antioxidant and ACE inhibitory activities were measured using the molecular weight cut-off fractions of the protamex hydrolysate
¹IC₅₀ values of ABTS⁺ scavenging, SOD-like, and ACE inhibitory activities were expressed as a mean ± SD
²The reducing power was expressed as an EC₅₀ value
³Means within the same row with different superscripts are significantly different by Duncan’s multiple range test (P < 0.05)
^A l-ascorbic acid was used as a positive control of ABTS⁺ radical scavenging and SOD-like activities, and reducing power
^BCaptopril was used as a positive control of ACE inhibitory activity

ISSN: 2234-1757