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Fig. 1 | Fisheries and Aquatic Sciences

Fig. 1

From: A single-step isolation of useful antioxidant compounds from Ishige okamurae by using centrifugal partition chromatography

Fig. 1

a HPLC and b ABTS+ online HPLC chromatogram of IOEA. Column: Atlantis T3 3 μm ODS column (150 mm × 4.6 mm i.d.); mobile phase: acetonitrile-water (0 → 40 min, 5:95 → 50:50 v/v; ~50 min, ~100:0 v/v; ~70 min, ~100:0 v/v); a flow rate of 200 μL/min, monitored at 230 nm. ABTS+ solution excreted at a flow rate of 700 μL/min and was measured at 680 nm absorbance. Peak I: DPHC, peak II: OPA

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