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Table 3 Expression stability ranking of 12 candidate reference genes during heavy metal exposures in the hepatopancreas of different treatment sets

From: Evaluation of reference genes for RT-qPCR study in abalone Haliotis discus hannai during heavy metal overload stress

RefFinder   geNorm   NormFinder   BestKeeper   Delta CT  
Genes GRV Genes SV (M) Genes SV Genes SD [±CP] Genes Ave. SD
RPL7 2.340 RPL8 0.059 RPL7 0.023 B-TU 0.100 RPL7 0.103
RPL3 2.991 ACTB 0.060 RPL3 0.047 RPL8 0.127 RPL3 0.113
RPL8 3.364 RPL4 0.061 EF1A 0.055 RPL4 0.129 EF1A 0.115
ACTB 3.600 RPL3 0.074 RPL5 0.058 ACTB 0.133 RPL5 0.117
RPL4 4.409 RPL7 0.079 RPL7A 0.069 RPL3 0.174 RPL7A 0.126
EF1A 4.695 EF1A 0.090 RPL4 0.101 RPL7 0.191 ACTB 0.135
RPL5 5.471 RPL5 0.096 ACTB 0.101 RPL7A 0.202 RPL4 0.136
RPL7A 6.117 RPL7A 0.101 RPL8 0.105 RPL5 0.225 RPL8 0.139
B-TU 6.447 UBE2 0.111 UBE2 0.116 EF1A 0.229 UBE2 0.146
UBE2 9.463 PPIB 0.119 PPIB 0.136 PPIB 0.257 PPIB 0.161
PPIB 10.000 GAPDH 0.127 GAPDH 0.156 UBE2 0.268 GAPDH 0.174
GAPDH 11.242 B-TU 0.139 B-TU 0.185 GAPDH 0.308 B-TU 0.199