Skip to main content

Advertisement

Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Table 2 Expression stability ranking of 12 candidate reference genes during heavy metal exposures in the gill of different treatment sets

From: Evaluation of reference genes for RT-qPCR study in abalone Haliotis discus hannai during heavy metal overload stress

RefFinder   geNorm   NormFinder   BestKeeper   Delta CT  
Genes GRV Genes SV (M) Genes SV Genes SD [±CP] Genes Ave. SD
RPL7 1.000 RPL8 0.199 RPL7 0.146 RPL7 0.117 RPL7 0.317
RPL8 1.682 RPL7 0.204 RPL8 0.150 RPL8 0.137 RPL8 0.320
ACTB 3.834 ACTB 0.209 RPL3 0.211 ACTB 0.169 RPL3 0.352
RPL3 4.559 RPL7A 0.236 ACTB 0.215 PPIB 0.188 PPIB 0.353
PPIB 4.681 PPIB 0.250 RPL7A 0.218 RPL7A 0.192 RPL7A 0.353
RPL7A 4.729 RPL3 0.266 PPIB 0.219 EF1A 0.199 ACTB 0.355
EF1A 6.735 EF1A 0.280 EF1A 0.227 RPL4 0.203 EF1A 0.360
RPL4 7.7737 RPL4 0.290 RPL4 0.231 RPL3 0.207 RPL4 0.364
GAPDH 9.000 GAPDH 0.304 GAPDH 0.282 GAPDH 0.280 GAPDH 0.397
RPL5 10.488 RPL5 0.329 UBE2 0.376 RPL5 0.316 UBE2 0.465
UBE2 10.719 UBE2 0.352 RPL5 0.408 B-TU 0.321 RPL5 0.479
B-TU 11.742 B-TU 0.392 B-TU 0.539 UBE2 0.341 B-TU 0.593