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Table 1 Summary on housekeeping reference genes and qPCR assay conditions used in this study

From: Evaluation of reference genes for RT-qPCR study in abalone Haliotis discus hannai during heavy metal overload stress

Gene

Description

Accession no.

Primer sequence (5′–3′)

Amplicon size (bp)

PCR (E)a

R 2 valueb

ACTB

Cytoskeletal β-actin

AY380809.1

FW: GGTATTGTTCTGGACTCTGG

162

1.968

0.996

  

RV: GGTGGTGGTGAATGAGTAAC

   

B-TU

β-tubulin

EF103431.1

FW: ACATTCACTAGGTGGGGGTA

161

1.976

0.998

  

RV: GTACTGACAATGTGGCGTTG

   

EF1A

Elongation factor-1 alpha

JX002677.1

FW: GCTCTCTGGAAGTTTGAGAC

165

1.974

0.994

  

RV: CTCCTTCGAGATACCAGCTT

   

GAPDH

Glyceraldehyde-3-phosphate dehydrogenase

ABO26632.1

FW: ACCGCTACACAGAAGACAGT

177

1.912

0.995

  

RV: TACATCAGGTACTGGGACAC

   

PPIB

Peptidyl-prolyl cis-trans isomerase B

KP698942

FW: CGAGAAAGCAGGACGAATTG

171

1.977

0.993

  

RV: AAGTCCCCTCCTTGGATCAT

   

RPL3

Ribosomal protein L3

KP698943

FW: TCATTGCACACACCCAGACT

168

1.911

0.997

  

RV: CAATGACCTCATCCTGTTCG

   

RPL4

Ribosomal protein L4

KP698944

FW: GCTGCTTCAAGACCGCTTAT

176

2.013

0.992

  

RV: TGGCCAGCTTTCTCTGAAAC

   

RPL5

Ribosomal protein L5

ABO26701.1

FW: AGATGAGGATGGCAAACCAG

168

1.937

0.996

  

RV: TCGCTGCTCTCAGAGTCAAA

   

RPL7

Ribosomal protein L7

KP698945

FW: CAAGCTGAACACTCCAAACG

156

1.997

0.994

  

RV: TCCACAGCACTGATGTTTCC

   

RPL7A

Ribosomal protein L7A

KP698946

FW: GCTGTCGAAAAAGGTTGAGC

165

1.972

0.995

  

RV: TGCTTCAAGACAGCGAACTG

   

RPL8

Ribosomal protein L8

KP698947

FW: TGGAAACTACGCCACAGTCA

161

1.944

0.995

  

RV: GTCCTGCCTTCAACATTGGT

   

UBE2

Ubiquitin-conjugating enzyme E2

KP698948

FW: CCAAGCTCTTCTTAGTGCAC

170

1.954

0.997

  

RV: CTCCCCACTTCCATCACTTT

   
  1. aPCR efficiency
  2. bDetermination coefficient
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Fisheries and Aquatic Sciences

ISSN: 2234-1757

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