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Fig. 4 | Fisheries and Aquatic Sciences

Fig. 4

From: Evaluation of reference genes for RT-qPCR study in abalone Haliotis discus hannai during heavy metal overload stress

Fig. 4

Validation of reference gene selection with the quantitative RT-qPCR assay of metallothionein (MT) transcripts in the gill (a) and hepatopancreas (b) subsets. MT expression levels in the Cd-exposed group were normalized against the most stable reference gene (R1), the two most stable references (R1+R2), the second least stable reference (R11), and the most unstable reference gene (R12). The statistical difference among the expression levels of MT transcripts calculated with different normalization genes is indicated based on ANOVA at P = 0.05

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