Table 1 Oligonucleotide primers used for the RT-PCR and quantitative real-time PCR to analyze mRNA expression of PLC-β1
Primer name | 5′-3′ sequence | Description |
|---|---|---|
β-actin-F | GACATGGAGAAGATCTGGCA | For RT-PCR and real-time qRT-PCR GenBank accession no AU090737 |
β-actin-R | ATGTCCTGCTCGAAGTCCAG | |
18 s rRNA-F | GTTGGTGGAGCGATTTGTCTGG | For RT-PCR and real-time qRT-PCR GenBank accession no DQ267937 |
18 s rRNA-R | CATCTAAGGGCATCACAGACCTG | |
IL-1β-F | AACAGCCAAGGCAAAGATTG | For RT-PCR and real-time qRT-PCR GenBank accession no AB070835 |
IL-1β-F | AATGTCCAGCTCCTCCTTCA | |
IL-6-F | TGGGCATCAACTCGGGCTAC | For RT-PCR and real-time qRT-PCR GenBank accession no DQ267937 |
IL-6-R | CGAAGGCCACAGATTGGTC | |
PoPLC-β1-F | CAACGTGATGGAGCAAAGAG | For RT-PCR and real-time qRT-PCR |
PoPLC-β1-R | GGTGTGTGGCTCAGTCCGTT |