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Table 1 Commercial protease inhibitory activities of PEG fractions obtained from the crude extracts of fish roes by the polyethylene glycol precipitation toward casein as a substrate

From: Recovery of serine protease inhibitor from fish roes by polyethylene glycol precipitation

  Fraction AP BH ST YT
SIA (U/mg) Recovery (%) SIA (U/mg) Recovery (%) SIA (U/mg) Recovery (%) SIA (U/mg) Recovery (%)
AL CE 235.0 100.0 40.4 100.0 925.6 100.0 144.0 100.0
PEG1 −44.9 −3.3 −537.2 −347.3 210.3 0.2 209.3 3.2
PEG2 −65.9 −0.6 −42.7 −2.5 180.6 1.2 85.8 1.8
PEG3 −23.4 −0.9 −4.7 −1.4 −160.1 −2.1 37.2 3.3
PEG4 −65.3 −0.8 28.4 10.6 −26.7 −0.4 18.5 1.6
BR CE 16.8 100.0 −58.7 100.0 9.9 100.0 16.2 100.0
PEG1 17.9 18.4 −93.1 41.4 72.6 6.8 45.7 6.2
PEG2 5.9 0.7 −23.0 0.9 −32.1 −20.2 −23.3 −4.2
PEG3 17.3 9.5 −30.1 6.3 −28.6 −35.5 −26.4 −20.6
PEG4 −14.1 −2.4 −19.4 5.0 −50.2 −62.5 −21.1 −15.7
PED CE 38.4 100.0 −54.5 100.0 35.6 100.0 112.5 100.0
PEG1 0.9 0.4 −66.2 31.7 618.6 16.1 566.2 11.0
PEG2 220.8 11.8 20.3 −0.9 50.6 8.9 137.0 3.6
PEG3 55.0 13.2 −42.9 9.6 9.3 3.2 50.5 5.7
PEG4 137.0 10.1 −23.2 6.4 15.8 5.5 22.4 2.4
CH CE 2183.9 100.0 672.5 100.0 1678.6 100.0 695.3 100.0
PEG1 673.4 5.3 −1477.5 −57.4 5768.6 3.2 2475.6 7.8
PEG2 1776.3 1.7 668.7 2.4 903.2 3.4 322.8 1.4
PEG3 767.7 3.2 288.4 5.2 1311.0 9.6 511.2 9.3
PEG4 9278.3 12.0 537.5 12.0 6687.0 49.1 3951.1 68.7
TR CE 73.1 100.0 74.5 100.0 81.4 100.0 44.3 100.0
PEG1 23.7 5.6 −990.1 −347.2 1999.7 22.7 −298.7 −14.8
PEG2 −259.4 −7.3 −416.9 −13.3 −513.2 −39.3 −299.0 −19.9
PEG3 −255.4 −32.2 −190.1 −31.2 302.8 45.7 118.9 33.9
PEG4 1170.9 45.2 98.2 19.8 2064.2 312.4 1536.2 419.2
  1. Minus (−) values are no protease inhibitory activity
  2. Recovery (%) = (total inhibitory activity of fraction/total inhibitory activity of CE) × 100
  3. PEG1–PEG4, 0–5, 5–10, 10–20, 20–40 % fractions obtained from polyethylene glycol-4000 precipitation
  4. AL alcalase, BM bromelain, PED papain-EDTA, CH chymotrypsin, TR Trypsin, CE crude extract, SIA specific inhibitory activity, RIA (%) relative inhibitor activity