Establishment and long-term culture of the cell lines derived from gonad tissues of Siberian sturgeon (Acipenser baerii)

Table 2 Information of Acipenser baerii gonad-derived cell lines established in this study

Name of cell line	Culture^a		Cryopreservation^b
Name of cell line	Passage no. in current	Culture period (day)	First passage no. cryopreserved	Final passage no. cryopreserved	Total no. of frozen vials stored
ABG1	80	504	9	80	46
ABG3	73	434	7	73	58
ABG5	80	405	8	80	39
ABG6	78	525	5	70	54
ABG7	83	402	6	83	62

^aComposition of culture media was Leibovitz’s L-15 medium supplemented with 20 % (v/v) fetal bovine serum and 1 % (v/v) penicillin and streptomycin
^bAll cell lines were frozen and stored in liquid nitrogen (−196 °C)

ISSN: 2234-1757