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Table 1 Primer sequences used in this study

From: Establishment and long-term culture of the cell lines derived from gonad tissues of Siberian sturgeon (Acipenser baerii)

Genes Primer sequences (5′ > 3′) Product size (bp) Accession number
ar Forward, TGAAGAAGATGAAGGGAGCAGAAGAT 231 DQ388357.1
Reverse, TCTCCCCCAGTTCATTCAAGC
cyp17a1 Forward, TCACACACTCCAGTATTGGTG 92 HQ026486.1
Reverse, CCATTCCTTTTCATCGTGATG
lh Forward, CTGCAGAGAAGGAGGAATGT 140 AJ251656.1
Reverse, GCGAAGATCCTTATAGGTGCA
sox9 Forward, AGCAGCAAAAACAAGCCTCA 113 EU241882.1
Reverse, AGCTCCGCGTTGTGAAGAT
star Forward, CAGAAGTCAATCAGCATCCT 79 FJ205610.1
Reverse, TCAGCACCTTGTCTCCATTG
β-actin Forward, CCCTGTTCCAGCCATCCTTC 155 JX027376.1
Reverse, GTCTGCAATGCCAGGGTACA
  1. Primer sequences for ar, cyp17a1, lh, sox9, and star genes were referred from Berbejillo et al. (2012)
  2. ar androgen receptor, cyp17a1 cytochrome P450, family 17, subfamily a, polypeptide 1, lh luteinizing hormone, sox9 sry-box containing gene 9, star steroidogenic acute regulatory protein