Fig. 1

Determination of optimal growth conditions for Acipenser baerii gonad-derived cell lines on temperature and fetal bovine serum (FBS) concentration. Three cell lines were cultured in 96-well microplates under different temperatures, and FBS concentrations and cell growth were measured on days 1, 3, 5, and 7. FBS concentration was fixed at 20 % in temperature test, and temperature was fixed at 28 °C in FBS concentration test. Significant high cell growth was observed in the cell groups cultured at 28 and 30 °C in all the three cell lines tested. In case of FBS concentration, 15 and 20 % FBS induced significant high cell growth in the ABG3 and ABG5 lines, while the ABG1 line showed the maximum cell growth under 20 % FBS. All data are mean ± SD of three independent experiments. Different letters indicate significant differences, p < 0.05