Temperature (°C) | Basal mediaa | No. of cell populations tested | No. (%)b of cell populations initially attached | No. (%)b of cell populations subcultured to | |||
---|---|---|---|---|---|---|---|
Passage 1 | Passage 2 | Passage 3 | Passage 4 | ||||
15 | L15 | 7 | 7 (100) | 7 (100) | 3 (43) | 1 (14) | 0 (0) |
DMEM | 7 | 7 (100) | 3 (43) | 1 (14) | 0 (0) | 0 (0) | |
20 | L15 | 7 | 7 (100) | 6 (86) | 3 (43) | 1 (14) | 0 (0) |
DMEM | 7 | 7 (100) | 3 (43) | 0 (0) | 0 (0) | 0 (0) | |
25 | L15 | 7 | 7 (100) | 5 (71) | 2 (29) | 1 (14) | 1 (14) |
DMEM | 7 | 7 (100) | 3 (43) | 0 (0) | 0 (0) | 0 (0) | |
Total | 42 | 42 (100) | 27 (64) | 9 (21) | 3 (7) | 1 (2) |