Temperature (°C)
|
Basal mediaa
|
No. of cell populations tested
|
No. (%)b of cell populations initially attached
|
No. (%)b of cell populations subcultured to
|
---|
Passage 1
|
Passage 2
|
Passage 3
|
Passage 4
|
---|
15
|
L15
|
7
|
7 (100)
|
7 (100)
|
3 (43)
|
1 (14)
|
0 (0)
|
|
DMEM
|
7
|
7 (100)
|
3 (43)
|
1 (14)
|
0 (0)
|
0 (0)
|
20
|
L15
|
7
|
7 (100)
|
6 (86)
|
3 (43)
|
1 (14)
|
0 (0)
|
|
DMEM
|
7
|
7 (100)
|
3 (43)
|
0 (0)
|
0 (0)
|
0 (0)
|
25
|
L15
|
7
|
7 (100)
|
5 (71)
|
2 (29)
|
1 (14)
|
1 (14)
|
|
DMEM
|
7
|
7 (100)
|
3 (43)
|
0 (0)
|
0 (0)
|
0 (0)
|
|
Total
|
42
|
42 (100)
|
27 (64)
|
9 (21)
|
3 (7)
|
1 (2)
|
-
aFor cell culture, basal media were supplemented with 20 % (v/v) fetal bovine serum and 1 % (v/v) mixed solution of penicillin and streptomycin
-
bPercentage of the number of cell populations tested