Isolation and in vitro culture of primary cell populations derived from ovarian tissues of the rockfish, Sebastes schlegeli

Table 1 Culture outcome of Sebastes schlegeli ovary-dissociated cells according to temperature and basal media

Temperature (°C)	Basal media^a	No. of cell populations tested	No. (%)^b of cell populations initially attached	No. (%)^b of cell populations subcultured to
Temperature (°C)	Basal media^a	No. of cell populations tested	No. (%)^b of cell populations initially attached	Passage 1	Passage 2	Passage 3	Passage 4
15	L15	7	7 (100)	7 (100)	3 (43)	1 (14)	0 (0)
	DMEM	7	7 (100)	3 (43)	1 (14)	0 (0)	0 (0)
20	L15	7	7 (100)	6 (86)	3 (43)	1 (14)	0 (0)
	DMEM	7	7 (100)	3 (43)	0 (0)	0 (0)	0 (0)
25	L15	7	7 (100)	5 (71)	2 (29)	1 (14)	1 (14)
	DMEM	7	7 (100)	3 (43)	0 (0)	0 (0)	0 (0)
	Total	42	42 (100)	27 (64)	9 (21)	3 (7)	1 (2)

^aFor cell culture, basal media were supplemented with 20 % (v/v) fetal bovine serum and 1 % (v/v) mixed solution of penicillin and streptomycin
^bPercentage of the number of cell populations tested