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Table 1 Culture outcome of Sebastes schlegeli ovary-dissociated cells according to temperature and basal media

From: Isolation and in vitro culture of primary cell populations derived from ovarian tissues of the rockfish, Sebastes schlegeli

Temperature (°C) Basal mediaa No. of cell populations tested No. (%)b of cell populations initially attached No. (%)b of cell populations subcultured to
Passage 1 Passage 2 Passage 3 Passage 4
15 L15 7 7 (100) 7 (100) 3 (43) 1 (14) 0 (0)
  DMEM 7 7 (100) 3 (43) 1 (14) 0 (0) 0 (0)
20 L15 7 7 (100) 6 (86) 3 (43) 1 (14) 0 (0)
  DMEM 7 7 (100) 3 (43) 0 (0) 0 (0) 0 (0)
25 L15 7 7 (100) 5 (71) 2 (29) 1 (14) 1 (14)
  DMEM 7 7 (100) 3 (43) 0 (0) 0 (0) 0 (0)
  Total 42 42 (100) 27 (64) 9 (21) 3 (7) 1 (2)
  1. aFor cell culture, basal media were supplemented with 20 % (v/v) fetal bovine serum and 1 % (v/v) mixed solution of penicillin and streptomycin
  2. bPercentage of the number of cell populations tested