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Table 2 Amino acid composition of the experimental diet (% of dry matter basis)

From: Determination of the dietary lysine requirement by measuring plasma free lysine concentrations in rainbow trout Oncorhynchus mykiss after dorsal aorta cannulation

Amino acids From casein and gelatin From crystalline amino acids Totala
EAA
 Arginine 0.353 1.924 2.277
 Histidine 0.194 0.725 0.919
 Isoleucine 0.252 1.674 1.926
 Leucine 0.493 2.702 3.195
 Lysine 0.502 0.218b 0.720
 Methionine 0.152 1.030 1.182
 Cystine 0.019 0.172 0.191
 Phenylalanine 0.271 1.742 2.013
 Tyrosine 0.270 1.335 1.605
 Threonine 0.221 1.601 1.822
 Tryptophan 0.065 0.462 0.527
 Valine 0.350 1.999 2.349
NEAA
 Alanine 0.345 1.741 2.086
 Aspartic acid 0.483 4.123 4.606
 Glycine 0.538 0.758 1.296
 Glutamic acid 1.298 4.459 5.757
 Proline 0.790 0.568 1.358
 Serine 0.374 2.398 2.772
  1. aThe amino acid profile was simulated with that of 35 % whole chicken egg protein
  2. bSeven experimental diets were formulated to have graded levels of lysine (0.72, 1.12, 1.52, 1.92, 2.32, 2.72 or 3.52 %); equal amount of aspartic acid and glutamic acid by weight were substituted for the proper amounts of lysine in the basal diet