Determination of the dietary lysine requirement by measuring plasma free lysine concentrations in rainbow trout Oncorhynchus mykiss after dorsal aorta cannulation

Table 2 Amino acid composition of the experimental diet (% of dry matter basis)

Amino acids	From casein and gelatin	From crystalline amino acids	Total^a
EAA
Arginine	0.353	1.924	2.277
Histidine	0.194	0.725	0.919
Isoleucine	0.252	1.674	1.926
Leucine	0.493	2.702	3.195
Lysine	0.502	0.218^b	0.720
Methionine	0.152	1.030	1.182
Cystine	0.019	0.172	0.191
Phenylalanine	0.271	1.742	2.013
Tyrosine	0.270	1.335	1.605
Threonine	0.221	1.601	1.822
Tryptophan	0.065	0.462	0.527
Valine	0.350	1.999	2.349
NEAA
Alanine	0.345	1.741	2.086
Aspartic acid	0.483	4.123	4.606
Glycine	0.538	0.758	1.296
Glutamic acid	1.298	4.459	5.757
Proline	0.790	0.568	1.358
Serine	0.374	2.398	2.772

^aThe amino acid profile was simulated with that of 35 % whole chicken egg protein
^bSeven experimental diets were formulated to have graded levels of lysine (0.72, 1.12, 1.52, 1.92, 2.32, 2.72 or 3.52 %); equal amount of aspartic acid and glutamic acid by weight were substituted for the proper amounts of lysine in the basal diet

ISSN: 2234-1757