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Table 1 Composition of the experimental diets (% of dry matter)a

From: Determination of the dietary lysine requirement by measuring plasma free lysine concentrations in rainbow trout Oncorhynchus mykiss after dorsal aorta cannulation

  Experimental diets (graded lysine level, % in diet)
Ingredients Diet 1 Diet 2 Diet 3 Diet 4 Diet 5 Diet 6 Diet 7
  (0.72) (1.12) (1.52) (1.92) (2.32) (2.72) (3.52)
EAAb 15.37 15.37 15.37 15.37 15.37 15.37 15.37
NEAAc 5.47 5.47 5.47 5.47 5.47 5.47 5.47
Caseind 5.00 5.00 5.00 5.00 5.00 5.00 5.00
Gelatined 2.00 2.00 2.00 2.00 2.00 2.00 2.00
Dextrind 27.97 27.97 27.97 27.97 27.97 27.97 27.97
Dextrosed 5.00 5.00 5.00 5.00 5.00 5.00 5.00
α-Cellulosed 8.20 8.20 8.20 8.20 8.20 8.20 8.20
Fish oile 10.00 10.00 10.00 10.00 10.00 10.00 10.00
Other ingredientsf 12.20 12.20 12.20 12.20 12.20 12.20 12.20
L-Lysineg 0.22 0.62 1.02 1.42 1.82 2.22 3.02
Aspartic acidg 4.12 3.92 3.72 3.52 3.32 3.12 2.72
Glutamic acidg 4.46 4.26 4.06 3.86 3.66 3.46 3.06
Total 100 100 100 100 100 100 100
  1. aDiets were neutralized with NaOH to give a final pH of 6.6
  2. bEAA, essential amino acids (g per 100 g diet): arginine, 1.924; histidine, 0.725; isoleucine, 1.674; leucine, 2.702; methionine, 1.030; cystine, 0.172; phenylalanine, 1.742; tyrosine, 1.335; threonine, 1.601; tryptophan,0.462; valine, 1.999 (Ajinomoto, Tokyo, Japan)
  3. cNEEA, Non-essential amino acids (g per 100 g diet): alanine, 1.741; glycine, 0.758; proline, 0.568; serine, 2.398 (Ajinomoto, Tokyo, Japan)
  4. dUnited States Biochemical (USB), Cleveland, Ohio, USA
  5. eEwha Oil Company, Busan, Korea
  6. fOther ingredients include: carboxymethyl cellulose, 1.00 % (United States Biochemical, Cleveland, Ohio, USA);Ca(H2PO4)2H2O, 3.00 %; choline bitartrate, 1.20 %(United States Biochemical, Cleveland, Ohio, USA), vitamin mixture, 3.00 % & mineral mixture, 4.00 % (Park et al. 2005)
  7. gAjinomoto, Tokyo, Japan