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Table 1 Composition of the experimental diets (% of dry matter)a

From: Determination of the dietary lysine requirement by measuring plasma free lysine concentrations in rainbow trout Oncorhynchus mykiss after dorsal aorta cannulation

 

Experimental diets (graded lysine level, % in diet)

Ingredients

Diet 1

Diet 2

Diet 3

Diet 4

Diet 5

Diet 6

Diet 7

 

(0.72)

(1.12)

(1.52)

(1.92)

(2.32)

(2.72)

(3.52)

EAAb

15.37

15.37

15.37

15.37

15.37

15.37

15.37

NEAAc

5.47

5.47

5.47

5.47

5.47

5.47

5.47

Caseind

5.00

5.00

5.00

5.00

5.00

5.00

5.00

Gelatined

2.00

2.00

2.00

2.00

2.00

2.00

2.00

Dextrind

27.97

27.97

27.97

27.97

27.97

27.97

27.97

Dextrosed

5.00

5.00

5.00

5.00

5.00

5.00

5.00

α-Cellulosed

8.20

8.20

8.20

8.20

8.20

8.20

8.20

Fish oile

10.00

10.00

10.00

10.00

10.00

10.00

10.00

Other ingredientsf

12.20

12.20

12.20

12.20

12.20

12.20

12.20

L-Lysineg

0.22

0.62

1.02

1.42

1.82

2.22

3.02

Aspartic acidg

4.12

3.92

3.72

3.52

3.32

3.12

2.72

Glutamic acidg

4.46

4.26

4.06

3.86

3.66

3.46

3.06

Total

100

100

100

100

100

100

100

  1. aDiets were neutralized with NaOH to give a final pH of 6.6
  2. bEAA, essential amino acids (g per 100 g diet): arginine, 1.924; histidine, 0.725; isoleucine, 1.674; leucine, 2.702; methionine, 1.030; cystine, 0.172; phenylalanine, 1.742; tyrosine, 1.335; threonine, 1.601; tryptophan,0.462; valine, 1.999 (Ajinomoto, Tokyo, Japan)
  3. cNEEA, Non-essential amino acids (g per 100 g diet): alanine, 1.741; glycine, 0.758; proline, 0.568; serine, 2.398 (Ajinomoto, Tokyo, Japan)
  4. dUnited States Biochemical (USB), Cleveland, Ohio, USA
  5. eEwha Oil Company, Busan, Korea
  6. fOther ingredients include: carboxymethyl cellulose, 1.00 % (United States Biochemical, Cleveland, Ohio, USA);Ca(H2PO4)2H2O, 3.00 %; choline bitartrate, 1.20 %(United States Biochemical, Cleveland, Ohio, USA), vitamin mixture, 3.00 % & mineral mixture, 4.00 % (Park et al. 2005)
  7. gAjinomoto, Tokyo, Japan