From: Method development and validation for dieckol in the standardization of phlorotannin preparations
Parameters | HPLC measurement conditions | ||
---|---|---|---|
Set 1 | Set 2 | Set 3 | |
Solutions | A: 15 vol% MeOH in deionized water, B: methanol | ||
Column | Shideido capcellpak C18 | Cadenza C18 | Supelco Discovery C18 |
Mobile phase (A:B) | 90:10 | 90:10 | 85:15 |
Flow rate (mL/min) | 0.8 | 0.8 | 0.7 |
Oven temperature (°C) | 35 | 35 | 25 |
Injection volume (μL) | 10 | 10 | 5 |
Elution time (min) | 60 | 60 | 55 |